real time quantitative reverse transcriptase polymerase chain reaction qrt pcr  (Bio-Rad)

Journal: Bioconjugate chemistry

Article Title: Delta-Opioid Receptor (δOR) Targeted Near-Infrared Fluorescent Agent for Imaging of Lung Cancer: Synthesis and Evaluation In Vitro and In Vivo

doi: 10.1021/acs.bioconjchem.5b00516

Figure Lengend Snippet: A) Normalized expression of OPRD1 based on Affymetrix microarray data. OPRD1 expression was analyzed for a panel of lung cancer cell lines and shown are the values for the positive and negative cell lines used in this work. Data are represented as mean ± SD (n = 3 for DMS-53 and n = 15 for H1299). **, p ≤ 0.01 B) The expression of OPRD1 was quantified by qRT-PCR and normalized to the expression of β-actin. The engineered cells are HCT-116 colorectal cancer cells that overexpress the delta opioid receptor (δOR). Also shown are the data for the parental HCT-116 cells that do not express the δOR. The endogenous cells are lung cancer cell lines that are positive (DMS-53) or negative (H1299) for OPRD1. Data are represented as mean ± SD (n = 3). **, p ≤ 0.01

Article Snippet: Determination of δOR mRNA expression in cell lines using Quantitative Real-Time Reverse-Transcriptase Polymerase Chain Reaction (qRT-PCR) RNA extractions were performed on cell lines using the RNeasy®Mini Kit (Qiagen, Cat. #74104) following the manufacturer’s instructions which include the DNase digestion steps.

Techniques: Expressing, Microarray, Quantitative RT-PCR